Covid-19

SARS Kits and VirSNiP Assays

RT-PCR Test Kits und VirSNiP Mutations Assays zur Nachverfolgung von Varianten

Zu Beginn des Jahres 2020 hat sich SARS-CoV-2 von Asien aus über die ganze Welt verteilt und ist immer noch eine Bedrohung. TIB Molbiol hat schon im Januar 2020 COVID-19 PCR Test Kits produziert und seither über 60 Millionen Tests ausgeliefert. Besondere Beachtung finden derzeit die verschiedenen Mutanten. Wir bieten VirSNiP Assays, um solche Mutationen zu entdecken. Diese Assays können auch in Routine Tests integriert werden.

Diese Seite enthält eine Übersicht der derzeit erhältlichen Tests. Aufgrund der dynamischen Entwicklung der Pandemie werden wir neue Tests entwickeln, wenn es nötig ist.

SARS RT-PCR test kits

FAM label Coronavirus detection

53-0775-96	Sarbeco N gene
53-0776-96	Sarbeco E gene
53-0777-96	SARS-CoV-2 RdRP

to be combined with an extraction control Cy5 channel

66-0909-96	EAV spiked target
66-0914-96	UBC human mRNA

90-9999-96	1-step RT polymerase (dry)

ModularDx kits are building blocks for multiplex PCR
One vial is for 96 reactions each 20 µl
Positive control is included
Polymerase and extraction kit not included

Product is dried - storage 4°C to 25°C
Shipping without temperature control
Frost exposure and temperature up to 45°C tested

IFU for use with Roche 480 instruments
FAM label TaqMan assays can be applied on any qPCR instrument (no instructions provided)

Standard run time approx 1 hour
FastCycling program 45 min run time

40-0776-96	Sarbeco E gene + EAV
90-9999-96	1-step RT polymerase

IFU for use with Roche 480 instruments:
FAM and Cy5 label TaqMan assays can be applied on any qPCR instrument minimum FAM and Cy5 channel (no instructions provided)

40-0770-96	Dual Target SARS + UBC
90-9999-96	1-step RT polymerase

Product evaluated with heat-treated gargle solution (no extraction required)

IFU for use with Roche 480 instruments Cyan500, FAM and HEX label TaqMan assays. Can be applied on any qPCR instrument minimum FAM and HEX channels (no instructions provided)

60-0770-96	Dual Target SARS + UBC

HEX	SARS-Cov-2 N gene
ROX	Sarbeco E gene
Cy5	UBC extraction control

Roche LightCycler® 480 II

50-0776-96	Sarbecovirus SARS-CoV-2
53-0101-96	Influenza A
58-0102-96	Influenza B
61-0110-96	RSV-A/B
64-0134-96	Picornavirus
66-0909-96	EAV extraction control

* IVD registration pending
Distributed by Roche Diagnostics

cobas z 480 Analyzer


53-0101-96	Influenza A
58-0102-96	Influenza B
61-0110-96	RSV-A/B
64-0776-96	SARS-CoV-2*
66-0909-96	EAV extraction control
70-0110-96	RSV-A/B*

SARS testing only (any instrument FAM and Cy5 channel)

50-0776-96	Sarbecovirus SARS-CoV-2
66-0990-96	EAV extraction control

50-0776-96	Sarbecovirus SARS-CoV-2
66-0914-96	UBC human mRNA

and Roche LightCycler^® Multiplex RNA Virus Master Cat.-No. 06 754 155 001 or

90-9999-96

1-step RT polymerase (dry)

Probe-based melting curve assay for testing for common mutations in the SARS genome, allowing to screen for emerging strains such as B.1.1.7 (UK variant).

The high melting peak is an indication of the presence of the variant of interest. The 'wild type' sequence shows a lower Tm peak; other variants are expected to yield even lower melting peaks.

IFU is for use with Roche 480 instruments. Any other instrument with a SYBR Green melting curve is capable to detect the melting curve; programming should be adapated to a heating rate approx 0.2°C / sec

TIB MOLBIOL VirSNiP Omicron Variant Assays (RUO)

Spike S371LS373P

Detects Omicron specifically (new mutations) -differentiates BA.1 / BA.2 (RUO)

Spike L452R + S371L S373P

Distinguishes Delta & Omicron in a single test - differentiates BA.1 / BA.2 (RUO)

Spike R346T

Detects Omicron (new mutation) BA.2.75.2, BA.4.6, BQ1.1 (RUO)

Spike E484A-F486S

Updated E484A assay, - detects Omicron by melting shift BA.1 / BA.4/.5 / BA.2.75.2 (RUO)

Spike N460K

Detects Omicron (new mutation) BA.2.75.2, XBB, BQ.1 (RUO)

Spike A67V del69/70

Updated del69/70 assay, - better resolution for BA. 1 deletion 69/70 (RUO)

VirSNiP strain identification integrated in routine testing

Roche 480	Instrument requirements : FAM (melting) / Cy5
50-0776-96	Sarbecovirus SARS-CoV-2
53-0780-96	SARS Spike N501Y (or other VirSNiP assay)
66-0990-96	EAV extraction control

Complete Kit	Instrument requirements : FAM (melting) / HEX / ROX / Cy5
53-0780-96	SARS Spike N501Y
60-0770-96	Dual Target SARS + UBC

FAM	Virus mutation
HEX	SARS-Cov-2 N gene
ROX	Sarbeco E gene
Cy5	UBC human extraction control

Complete Kit	Instrument requirements : FAM (melting) / HEX
53-0780-96	SARS Spike N501Y
40-0770-96	Dual Target SARS + UBC

FAM	Virus mutation
HEX	UBC human extraction control

Winterplex®	Instrument requirements : 500 / FAM (melting) / HEX / ROX / 640 / Cy5
50-0776-96	Sarbecovirus SARS-CoV-2
53-0780-96	SARS Spike N501Y (or other VirSNiP assay)
53-0101-96	Influenza A
58-0102-96	Influenza B
61-0110-96	RSV-A/B
64-0134-96	Picornavirus

66-0990-96	EAV extraction control